EGFR L858 R Mutation Detection Kit

Description

iGentBio quantitative PCR platform allows the simultaneous amplification and detection of nucleic acids within a closed reaction vessel. The method is based upon the incorporation of energy transfer-labeled primers into the amplification product. The unique 3D folding structure of the primer blocks the fluorescent signal generation via energy transfer. The fluorescent signal is produced only when the primer is incorporated into a PCR product. The increment of the fluorescence signal directly correlates with the accumulation of PCR product at each cycle. The folded primers have an extremely low fluorescence eliminating the need to purify the PCR product prior to quantification; therefore, PCR and fluorescent signal detection can occur in a single reaction vessel. Signal is measured either during the reaction (real-time) or after the last cycle of the reaction (endpoint). Multiple fluorescent dyes can be associated with different primers and are suitable for SNP detection for both single nucleotide and In/Del mutations.  Melting curve analysis can also be used as a confirmative data to identify the truly positive alleles and eliminate the fail positive that is caused by the primer dimer  iGentbio EGFR L858R kit is designed to detect one of EGFR mutations which have been reported to associated with the sensitivity of anti-EGFR inhibitors (TKIs). L858R mutation is located on exon 21. The others are located through exons 18 to 21. These mutations affect the adenosine triphosphate (ATP)-binding. In vitro studies have shown that EGFR mutants have constitutive TK activity and, therefore, a greater sensitivity to anti-EGFR inhibition. Mutations in the kinase domain of EGFR have also been associated with an acquired resistance to EGFR TKIs and result in TKI drugs with less sensitivity This product can be used to test patient sample DNA from tissue sample or from whole blood cells. The result will genotype both wide type and mutant. This product can’t be used alone for diagnostic purpose. RESERCH USE ONLY.