iPSC Generation

Footprint-Free iPSC Generation Service

Induced pluripotent stem cells have revolutionized in vitro disease modeling and hold enormous promise as an ethical and individualized source of stem cells for therapeutic use. Normally somatic cells are “reprogrammed” back to a pluripotent state using a virus to transiently express key transcription factors. Unfortunately this method will leave viral remnants in the DNA of iPSC lines. The scientists at iGentBio use only footprint-free methods to generate iPSC lines 100% free of genomic contamination. Don’t let the wrong reprogramming method invalidate your scientific results or worse! Contact us today for a personalized quote.

Key Benefits and Deliverables
For each patient sample you receive:

  • • 3 clones guaranteed Footprint-Free
  • • G-banding and karyotyping
  • • SNP panel cell line barcoding
  • • Pluripotent marker antibody staining
  • • Pluripotent gene expression profiling
  • • Embryoid body formation and gene expression profiling
  • • Guaranteed contamination free

Cell Types for iPSC Derivation
Cell types includes:

  • • Skin Fibroblast
  • • PBMC
  • • EBV-transformed Lymphocytes
  • • ADSC
  • • MSC
  • • HUVEC

Note: Cells should be at low passage and able to proliferate with a doubling time of 12-24 hours. Slowing dividing cells tend to have low reprogramming efficiency, hence such reprogramming cannot be guaranteed.